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Fig. 2 | Parasites & Vectors

Fig. 2

From: A duplex tetra-primer ARMS-PCR assay to discriminate three species of the Schistosoma haematobium group: Schistosoma curassoni, S. bovis, S. haematobium and their hybrids

Fig. 2

Virtual gels of 18S T-ARMS-PCR (a) internal transcribed sequence (ITS) T-ARMS-PCR (b) and duplex T-ARMS-PCR (c). The 382- and 667-bp amplicons are the internal positive controls for the Schistosoma sp. 18S and ITS DNA regions, respectively. The 18S T-ARMS-PCR was designed to distinguish Schistosoma bovis (Sb) from Schistosoma curassoni (Sc) or Schistosoma haemobium (Sh), whereas the ITS T-ARMS-PCR was designed to distinguish Sh from Sb or Sc. When combined in duplex, the ITS/18S T-ARMS-PCR should provide unique amplicon profiles for each species (Sh: 120, 382, 487 and 667 bp; Sc: 120, 234, 382 and 667 bp; Sb: 234, 316, 382, and 667 bp) and/or hybrid forms (Sb×Sc: 120, 234, 316, 382 and 667 bp; Sh×Sc: 120, 234, 382, 487 and 667 bp; and Sh×Sb: 120, 234, 316, 382, 487 and 667 bp). C+ Internal positive control

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Parasites & Vectors

ISSN: 1756-3305

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