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Fig. 2 | Parasites & Vectors

Fig. 2

From: Investigating potential sand fly vectors after the first reported outbreak of cutaneous leishmaniasis in Ghana

Fig. 2

a Detection of Leishmania DNA in Sergentomyia ingrami pools using internal transcribed spacer region 1-polymerase chain reaction. M 100-base pair marker, lane 1 positive control with 5 µg DNA from Leishmania tropica, lane 2 positive control with 5 µg DNA from Leishmania major, lane 3 amplified Leishmania DNA within the human blood-fed S. ingrami pool, lanes 4 and 5 amplified Leishmania DNA within unfed S. ingrami pools, lane 6 negative control. b Polymerase chain reaction-restriction fragment length polymorphism analysis of internal transcribed spacer region 1 genes obtained with HaeIII. M 100-base pair marker, lane 1 L. tropica reference strain, lane 2 L. major reference strain, lanes 3–5 S. ingrami pools

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