Fig. 4

Co-infection of Ae. aegypti mosquitos with DENV and ZIKV results in differential viral genome expression. A Time course of experimental oral challenge with DENV2 and ZIKV. Mosquitos were captured 12 h ahead of day 0 and presented with blood meal (BM), DENV2, ZIKV, or maintained on sugar feeding (Sugar). On day 5, one group was given a second BM (BM-BM), and others were challenged with DENV2 or ZIKV. At 7 dpi (day 12), whole mosquito bodies were collected and homogenized. Relative viral genome expression of B DENV2 and C ZIKV was determined by qRT-PCR analysis, with normalization to the endosomal S7 protein. Each of six oral challenge schemes consisted of at least five biologically independent cohorts, and post hoc comparisons between groups were performed using Tukey’s multiple comparisons test; **P < 0.01; ***P < 0.001; ****P < 0.0001