Fig. 9

Schematic of the HbARI locus highlighting that the annotations in H. bakeri still need work. The two genome assemblies for H. bakeri, PRJEB1203 and PRJEB15396 (shown collectively as a black line), agree in this region with the exception of the area shown in the black box. The conflicting annotations for HbARI, HPBE_0000813301 (teal) and HPOL_0001636401 (green) are shown in their annotated positions and drawn to scale. HPOL_0001636401 is shorter at the 5′ end and consequently does not contain the signal peptide predicted to be in HPBE_0000813301. Since we know HbARI is a secreted protein because it was identified among HES, the PRJEB15396 annotation must be incorrect. As it is, HPOL_0001636401 is predicted to localize to the mitochondrion (data not shown). The coverage of all of the RNA-seq reads generated in this study in this region is shown as a line graph above the annotations. The HPBE_0000813301 annotation contains an extra 5′ exon that has no support in any of the RNA-seq datasets