Fig.5

Effects of KNK437 on the GLV expression level. The expression level of GLV capsid protein treated with KNK437 at different concentrations (25 μM, 50 μM, 75 μM and 100 μM) was measured by qPCR, with three replicates of each concentration. The data of GLV capsid protein mRNA were normalized to Gd actin. The cDNA concentration of Giardia trophozoites was the same in each group. Primers of qPCR are listed in Additional file 1: Table S2. Results are expressed as mean ± SD from three separate experiments. ns, no significant difference, *P < 0.05, **P < 0.01, ***P < 0.001, ****p < 0.0001