Fig. 3

Validation of the relationship between miR-29a-3p and Robo1. A, B LX-2 cells were exposed to 10 ng/ml TGF-β1 for 48 h, and the expression of miR-29a-3p and Robo1 was detected by qPCR (n = 3). C–F LX-2 cells were cultured on a plastic plate and transfected with 50 nM miR-29a-3p mimics, 100 nM miR-29a-3p inhibitors, or their negative controls (NC) for 48 h. The expression of miR-29a-3p and Robo1 was determined by qPCR (n = 3) (C, D) and western blotting (n = 3–4) (E, F). Data are presented as the mean ± SD. All data are representative of at least three independent experiments. Significance was determined by the two-tailed Student’s t test (A–D, F). *P < 0.05, **P < 0.01, ***P < 0.001. miR-29a-3p: microRNA-29a-3p; Robo1: Roundabout homolog 1