Fig. 5

MIR29A mice were better able to prevent HSC activation during schistosome infection. A The expression level of Robo1 in livers was determined by qPCR (n = 7). B, C Robo1 protein was determined by western blotting, quantified using ImageJ, and normalized to GAPDH (n = 6). D–H Primary HSCs were isolated from the liver tissues of mice. D Representative immunofluorescence staining of Robo1 (green) colocalization with α-SMA (red) in primary HSCs was detected. Insets show a higher magnification of the outlined area. Scale bar, 50 μm. E–H The expression of Col1α1, Col3α1, α-SMA, and TGF-β1 in primary HSCs during infection was detected by qPCR (n = 7). Data are presented as the mean ± SD from two independent experiments. Significance was determined by one-way ANOVA with Tukey’s correction for comparisons between two groups (A, C, E–H). ^##P < 0.01, compared with uninfected WT samples. **P < 0.01, ****P < 0.0001, compared with infected WT samples. miR-29a-3p: microRNA-29a-3p; Robo1: Roundabout homolog 1