Fig. 7

miR-29a-3p agomir-mediated elevation of miR-29a-3p prevented HSC activation during schistosome infection. A, B miR-29a-3p and Robo1 expression levels in livers were determined by qPCR (n = 3–4). C, D Robo1 protein was determined by western blotting, quantified using ImageJ, and normalized to GAPDH (n = 3). E–I Primary HSCs were isolated from the liver tissues of mice. E Representative immunofluorescence staining of Robo1 (green) colocalization with α-SMA (red) in primary HSCs. Scale bar, 100 μm. F–I The expression of Col1α1, Col3α1, α-SMA, and TGF-β1 in primary HSCs during infection was detected by qPCR (n = 3–4). Data are presented as the mean ± SD from two independent experiments. Significance was determined by one-way ANOVA with Tukey’s correction for comparisons between two groups (A, B, D, F–I). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. miR-29a-3p: microRNA-29a-3p; Robo1: Roundabout homolog 1