Fig. 8

Overexpression of miR-29a-3p attenuated HSC activation by inhibiting Robo1 pathways. LX-2 cells were pretreated with 50 nM miR-29a-3p mimics or their negative controls for 6 h, TGF-β1 (10 ng/ml) was added to the medium, and the expression levels of fibrosis markers and Robo1 were determined after 48 h of incubation. A–E The expression of Col1α1, Col3α1, α-SMA, TGF-β1, and Robo1 in LX-2 cells was detected by qPCR (n = 3–6). F Robo1 protein was determined by western blotting, quantified using ImageJ, and normalized to GAPDH (n = 4). Data are presented as the mean ± SD of three independent experiments. Multiple comparisons were performed by one-way ANOVA with Tukey’s correction for comparisons between two groups (A–F). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. miR-29a-3p: microRNA-29a-3p; Robo1: Roundabout homolog 1