Table 2 Primer sequences used in this study
Gene | Species | Primer sequence (5′-3′) | Size (bp) |
|---|---|---|---|
Robo1 | Human | F: CGCCCCACACCCACTATTG R: GAAGTCATCCCGAAGTATGG | 237 |
Col1α1 | Human | F: GAAGACATCCCACCAATCACC R: TCTCGTCACAGATCACGTCATC | 136 |
Col3α1 | Human | F: CTACTTCTCGCTCTGCTTCATC R: CACAGACACATATTTGGCATGG | 136 |
α-SMA | Human | F: GTCCCACATCAGGGAGTAA R: TCGGATATTCAGCGTCAGGA | 185 |
TGF-β1 | Human | F: TGGCGATACCTCAGCAACC R: CTAAGGCGAAAGCCCTCAAT | 168 |
GAPDH | Human | F: GGAGCGAGATCCCTCCAAAAT R: GGCTGTTGTCATACTTCTCATGG | 197 |
Robo1 | Mouse | F: GAGCCTGCTCACTTTTACCTC R: GGTCTGAAGGGTGTTCAACAAT | 184 |
Col1α1 | Mouse | F: CGCCATCAAGGTCTACTGC R: ACGGGAATCCATCGGTCA | 152 |
Col3α1 | Mouse | F: GCCCACAGCCTTCTACACCT R: GCCAGGGTCACCATTTCTC | 110 |
α-SMA | Mouse | F: GAAGTATCCGATAGAACACG R: CTCAAACATAATATGGGTCA | 185 |
TGF-β1 | Mouse | F: TGACGTCACTGGGGGTTGTACC R: GGTTCATGTCATGGATGGTGC | 183 |
GAPDH | Mouse | F: CCTCGTCCCGTAGACAAAATG R: TGTAGTTGAGGTCAATGAAGGG | 139 |