Fig. 4

Detection of anti-rHlFBA (recombinant fructose-1,6-bisphosphate aldolase from Haemaphysalis longicornis) antibody level in rabbit serum by enzyme-linked immunosorbent assay. Arrows indicate the days of immunization days. Different lowercase letters represent significant differences across the group at the indicated time point (P < 0.05). Red circles, blue squares and black triangles represent the OD values detected by ELISA in the HlFBA group, Trx group, and PBS group at different time points, respectively. All analyses were performed using three technical and three biological replicates