Molecular characterization and immune efficacy of fructose-1,6-bisphosphate aldolase from Haemaphysalis longicornis (Acari: Ixodidae)

Table 1 Effect of vaccination with recombinant fructose-1,6-bisphosphate aldolase protein from Haemaphysalis longicornis on tick infestations

Trial	Total number of female ticks	Number of biting ticks	Engorgement weight (mg)	Oviposition (mg)	Hatchability (%)	E_W^a	E_O^a	E_H^a	E (%)^b
Phosphate-buffered saline (PBS) group (n = 6)	276	188	193.56 ± 22.80a	73.52 ± 18.85a	78.44 ± 4.52a	–	–	–
Thioredoxin (Trx) group (n = 6)	276	218	183.48 ± 19.41a	66.36 ± 14.76a	78.56 ± 2.77a
rHlFBA group (n = 6)	276	213	142.10 ± 25.09b	36.12 ± 13.68b	59.65 ± 1.86b	0.77	0.54	0.76	68.4

Data on engorgement weight, oviposition and hatchability are presented as the mean ± standard error. Values followed by different lowercase letters in columns represent significant differences between the group (P < 0.05)
rHlFBA, Recombinant fructose-1,6-bisphosphate aldolase from Haemaphysalis longicornis
^aFormulas for the calculation of reduction in engorgement weight (E_W), oviposition (E_O) and hatchability (E_H) are described in the Methods section
^bEfficacy (E) = 100 [1- (E_W × E_O × E_H)]. The number of rabbits immunized in each group is six (n = 6)

ISSN: 1756-3305