Fig. 4

Interaction between TvAP33 protein and molecules in host cells. a Immunofluorescence assay of TvAP33 binding to VK2/E6E7 cells. The VK2/E6E7 cells were cocultured with the final concentration of 20 μg/ml of rTvAP33 at 37 °C and 5% CO₂ for 30 min, and the same concentration of pET-32a protein was used as the control. Cy3 dye (red) was used to label TvAP33 protein bound to VK2/E6E7 cells and DAPI (blue) was used to stain the nuclei. b Preliminary screening of molecules interacting with TvAP33 on VK2/E6E7 cells by the yeast two-hybrid system. c Second screening by yeast two-hybrid