Table 1 The sequences of oligonucleotide primers and small interfering RNAs
Name | Sequence (5′ → 3′) | Description |
|---|---|---|
TvAP33 sense | GGCACATCCGAAGAAGATGC | Oligonucleotide primer sequences used for detecting the mRNA level of TvAP33 by qPCR in this research |
TvAP33 antisense | CTTGCCTGGTGGAGCTGTA | |
TvActin sense | TCACAGCTCTTGCTCCACCA | |
TvActin antisense | AAGCACTTGCGGTGAACGAT | |
BNIP3 sense | CAGCATGAGTCTGGACGGAG | Oligonucleotide primer sequences used for detecting the mRNA level of BNIP3 by real-time PCR in this research |
BNIP3 antisense | GCCGACTTGACCAATCCCAT | |
Actin sense | CGTGACATTAAGGAGAAGCTG | |
Actin antisense | CTAGAAGCATTTGCGGTGGAC | |
TvAP33-siRNA sense | GUCUCCCAAUCUUCAAGAACATT | The siRNA sequences used for knocking down the expression of TvAP33 in T. vaginalis trophozoites |
TvAP33-siRNA antisense | UGUUCUUGAAGAUUGGGAGACTT | |
TvNC sense | CGUCCCGUAGCCCACUAAAdTdT | |
TvNC antisense | UUUAGUGGGCUACGGGACGdTdT | |
BNIP3-siRNA sense | GCCUCGGUUUCUAUUUAUAAUTT | The siRNA sequences used for knocking down the expression of BNIP3 in VK2/E6E7 cells |
BNIP3-siRNA antisense | AUUAUAAAUAGAAACCGAGGCTT | |
NC sense | UUCUCCGAACGUGUCACGUdTdT | |
NC antisense | ACGUGACACGUUCGGAGAAdTdT |