Amplicon sequencing allows differential quantification of closely related parasite species: an example from rodent Coccidia (Eimeria)

Table 2 Detecting Eimeria sensitivity and specificity for sequencing of Eimeria ASVs produced with standard PCR and microfluidic PCR. Eimeria detection with qPCR (genome copies per nanograms of DNA) is the reference standard

	All Eimeria ASVs (%)	ASV1 (%)	ASV2 (%)
Sequenced with standard PCR
Sensitivity	93.8	83.3	92.6
Specificity	64.9	89.2	64.9
Sequenced with microfluidic PCR
Sensitivity	78.5	69.9	73.6
Specificity	94.1	98.0	94.1

ISSN: 1756-3305