Genetic diversity of vector-borne pathogens in ixodid ticks infesting dogs from Pakistan with notes on Ehrlichia canis, Rickettsia raoultii and Dirofilaria immitis detection

Table 1 List of primer sets used for the detection/amplification of V/TBP genes in ixodid ticks

Desired organism	Target gene	Primer	Primer sequence (5′–3′)	Amplicon size (bp)	References
Anaplasma/Ehrlichia species	16S rRNA	EHR16SF	GGT ACC YAC AGA AGA AGT CC	~ 345	[43]
Anaplasma/Ehrlichia species	16S rRNA	EHR16SR	TAG CAC TCA TCG TTT ACA GC	~ 345	[43]
Rickettsia species	16S rRNA	Rick-F1	GAA CGC TAT CGG TAT GCT TAA CAC A	~ 364	[44]
	16S rRNA	Rick-R2	CAT CAC TCA CTC GGT ATT GCT GGA	~ 364	[44]
	gltA	CS-78	GCA AGT ATC GGT GAG GAT GTA AT	~ 401	[45]
	gltA	CS-238	GCT TCC TAA AAT TCA ATA AAT CAG GAT	~ 401	[45]
Babesia/Theileria species	18S rRNA	NWF	GTC TTG TAA TTG GAA TGA TGG	~ 500	[46]
Babesia/Theileria species	18S rRNA	NWR	TAG TTT ATG GTT AGG ACT ACG	~ 500	[46]
Dirofilaria species	cox1	LCOI490	GGT CAA CAA ATC ATA AAG ATA TTG	~ 710	[47]
Dirofilaria species	cox1	HCO2198	TAA ACT TCA GGG TGA CCA AAA AAT CA	~ 710	[47]

16S rRNA: 16S ribosomal RNA gene; 18S rRNA: 18S ribosomal RNA gene; gltA: Rickettsial citrate synthase-encoding gene; cox1: Cytochrome c oxidases subunit 1 gene

ISSN: 1756-3305