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Table 2 Molecular detection of V/TBPs in hard tick species collected from dogs across central Khyber Pakhtunkhwa, Pakistan

From: Genetic diversity of vector-borne pathogens in ixodid ticks infesting dogs from Pakistan with notes on Ehrlichia canis, Rickettsia raoultii and Dirofilaria immitis detection

Study area (districts)

Ixodid tick species

Canine associated vector/tick-borne pathogens

Ticks screened n/t (%)

Genus Ehrlichia

Genus Rickettsia

Genus Theileria

Genus Dirofilaria

Genus Wolbachia

E. canis

Ehrlichia sp.

R. massiliae

R. raoultii

Rickettsia sp.

T. annulata

D. immitis

Wolbachia sp.

Charsadda

Rh. sanguineus s.l.

 + 

 + 

 + 

 + 

 + 

 − 

 + 

 + 

20/20 (100%)

Malakand

Rh. turanicus s.s.

 + 

 + 

 + 

 + 

 + 

 − 

 + 

 + 

13/20 (65%)

Mardan

Rh. haemaphysaloides

 + 

 + 

 + 

 − 

 + 

 + 

 − 

 − 

6/20 (30%)

Peshawar

Rh. microplus

 − 

 − 

 + 

 − 

 − 

 − 

 − 

 − 

1/20 (5%)

Swabi

Hy. dromedarii

 − 

 − 

 + 

 − 

 + 

 + 

 − 

 − 

8/20 (40%)

 

Hy. excavatum

 − 

 − 

 + 

 − 

 − 

 + 

 − 

 − 

2/20 (10%)

Total n (%)

V/TBP Sp

13 (10.8%)

4 (3.3%)

23 (19.5%)

8 (6.7%)

9 (7.5%)

7 (5.8%)

7 (5.8%)

5 (4.2%)

 

V/TBP G

17 (14.1%)

40 (33.7%)

7 (5.8%)

7 (5.8%)

5 (4.2%)

50/120 (41.7%)

  1. n/t (%): n: number of positive ticks, t: total ticks screened, %: percent positive tick; n (%): n: number of positive tick species, %: pathogen/endosymbiont prevalence in all tick species; V/TBP Sp./G.: vector/tick-borne pathogen species/genus prevalence; +/−: positive/negative