Fig. 5

High-resolution melt curves with pan-nematode ITS-2 primers. Example samples include A a H. contortus-dominant infection with a single peak at 79.77 °C ± SD 0.04 °C, B a Trichostrongylus-dominant infection with peaks at 78.63 °C ± SD 0.027 °C and 81.6 °C ± SD 0.052 °C, and C a mixed infection with a H. contortus-associated peak at 79.63 °C ± SD 0.113 °C and a second Trichostrongylus-associated peak at 81.48 °C ± SD 0.04 °C. Insets show relative species abundance (as percentage) from EP  endpoint PCR, SP singleplex PCR, MP multiplex PCR, and Q qPCR analyses where H con  H. contortus, T col  T. colubriformis, O col  O. columbianum