Low-cost molecular methods to characterise gastrointestinal nematode co-infections of goats in Africa

Parasites & Vectors

Table 1 Costs of molecular methods tested

Method	Species tested	Targets/RXN (reaction)	Replicates/RXN	Thermocycler	Throughput/run^a,c	Cost/RXN ^b	Cost/sample^b,c	Cost/run^b,c
Endpoint PCR	12	1	1	96-well MiniAmp Plus	32	£0.21	£0.63	£20.16
Singleplex PCR	4	1	1	96-well MiniAmp Plus	32	£0.21	£0.63	£20.16
Multiplex PCR	3	3	1	96-well MiniAmp Plus	96	£0.21	£0.21	£20.16
qPCR	2	1	3	72-tube Rotor-Gene Q	8	£0.53	£4.73	£37.80
HRMC	Various	Various	3	72-tube Rotor-Gene Q	24	£0.53	£4.73	£37.80

Reagent costs for methods trialled in the studies listed include the number of species targets and the throughput using equipment listed in the table. Consumables and equipment costs such as pipette tips and labour costs are not included
^aNumber of samples processed per run when testing for three species, excluding controls
^bCost of PCR reagents as described in the Methods section
^cCost calculated to check for three species per sample

ISSN: 1756-3305