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Table 1 Average group results with standard deviations of Dirofilaria immitis antigen testing using DiroCHEK® (Zoetis Animal Health, New Jersey, USA)—optical density (OD) readings were obtained at the time of result interpretation for both pre-heat and post-heat treatment of samples—and microfilaria testing (microfilaria [mf] per ml of blood) which occurred before each mosquito challenge

From: Fluralaner (Bravecto®) treatment kills Aedes aegypti after feeding on Dirofilaria immitis-infected dogs

Study day

 

Average antigen ODa

Average mf/mlc

  

Pre-heat treatment

Post-heat treatment

 

−8

    
 

Control

0.25 ± 0.11

0.93 ± 0.39

18,383.3 ± 4776.8

 

Fluralaner

0.18 ± 0.12

0.78 ± 0.07

20,215.0 ± 3.530.4

1

    
 

Control

NAb

NAb

NAb

 

Fluralaner

NAb

NAb

NAb

29

    
 

Control

0.22 ± 0.12

0.70 ± 0.11

23,306.7 ± 6595.3

 

Fluralaner

0.32 ± 0.12

0.65 ± 0.07

18,512.5 ± 14,032.8

55

    
 

Control

0.15 ± 0.04

0.76 ± 0.05

30,606.7 ± 10,717.2

 

Fluralaner

0.18 ± 0.10

0.71 ± 0.14

30,285.0 ± 18,255.8

83

    
 

Control

0.33 ± 0.10

0.92 ± 0.07

32,410.0 ± 20,933.2

 

Fluralaner

0.44 ± 0.15

0.93 ± 0.03

21,750.0 ± 13,970.6

  1. NA not applicable
  2. aNegative control value subtracted from each sample OD reading before averages were calculated
  3. b Testing was not performed since results were obtained less than 10 days earlier
  4. cOnly three of the four control dogs became microfilaremic; the amicrofilaremic dog was excluded from these calculations. For more information on the occult dog, please see the Discussion