Table 1 Microsatellite sequences identified in the nuclear genome of Aedes koreicus, and organisation of the validated markers as multiplex PCR
From: Development of microsatellite markers for the invasive mosquito Aedes koreicus
Locus name | Reference sequence (Run number.read number) | (Repeat motif)na | Primer sequence (5′-3′) | PCR fluorophoresb | Size range (bp) | |
|---|---|---|---|---|---|---|
Multiplex A | AK01 | ERR7598207.14364316 | (CAA)7 | F: GCCGCTACAAACAGCTCAGT R: TCGTAAATTCGACGGGGTAG | TAMRA | 121–127 |
AK05 | ERR7598207.14346661 | (CAA)6 | F: AACCGACTACGATGCACACA R: TAAACACAGAGCCAGCCACA | HEX | 104–116 | |
AK07 | ERR7598207.13874909 | (GGC)6 | F: ATCGAGCACACCGTCGTT R: ATCCATTTTTCTTTCTTTCGCTGT | 6-FAM | 144–150 | |
AK13 | ERR7598207.13190639 | (GTT)6 | F:CCATTAAAAGGTTTTACTCAATGTTGT R: AAGAGACGGCTGTGGTCCT | TAMRA | 141–144 | |
Multiplex B | AK03 | ERR7598207.14368852 | (GAA)6 | F: CGACTCCCTGAGCATAGTGT R: CATGCTATGATGAGCACATTGA | 6-FAM | 133–139 |
AK09 | ERR7598207.14366337 | (ACG)11 | F: TACCCGAATCCAGCAAACAT R: GCCAGAAGGACATCGTCAC | TAMRA | 90–102 | |
AK10 | ERR7598207.14369161 | (ATC)6 | F: AACTTGCCGCAATAGATGGT R: CCAAGACCTATTCTGGAAGCA | HEX | 117–126 | |
AK12 | ERR7598207.14357357 | (ACA)6 | F: CCAAAACGTATATCATCCGAAG R: GGAGAATCATCTGTGATAGTTTTGTG | 6-FAM | 91–97 | |
Multiplex C | AK15 | ERR7598207.14247464 | (TAA)7 | F: CCCAGTCCCAGCTTGAATAA R: AGGCATCAAGCTGCATCCTA | HEX | 103–106 |
AK23 | ERR7598207.14317350 | (CCTT)5 | F: GGGGTTAACCAACCGAACC R: CGTTTGCCTTATACTGACAAATCT | 6-FAM | 134–145 | |
AK28 | ERR7598207.14270387 | (GGTT)8 | F: GCAGCAACTTCCTTCCGTAG R: AACTGGCTGACCAGCGTAAC | 6-FAM | 80–104 | |
Discarded | AK02 | ERR7598207.1435939 | (GCA)10 | F: GGTGCGTCAGCAGCAGTAGT R: GGGATGAGAGACAGAGGGAAA | ||
AK04 | ERR7598207.14356098 | (TGA)8 | F: GATTAGATAATCCGGCATGGA R: TTAAGATCAACCAGTGGCATAAGA | |||
AK06 | ERR7598207.14366465 | (CGT)6 | F: CTCCGCCACCATTTTAGTGT R: TCCAGTTTTTCAAGAGCCATA | |||
AK08 | ERR7598207.12531158 | (GGA)7 | F: GCAGCCGGTAGTGGTGAC R: GCAGAATTGCTTGCTGCAC | |||
AK11 | ERR7598207.14345973 | (AAT)6 | F: TACCCGAATCCAGCAAACAT R: GCCAGAAGGACATCGTCAC | |||
AK14 | ERR7598207.14353635 | (GAT)8 | F: CGGCTTTGATGACAGGAAAC R: TGTTTACCAGACCGCACTGT | |||
AK16 | ERR7598207.14084369 | (GAA)6 | F: ACAATCCCTGGCAATGTCC R: CAGCCTGCACAAACAACAC | |||
AK17 | ERR7598207.14057111 | (TCT)6 | F: AGCAATGATGGGTGACTATTGTT R: AGCAATGATGGGTGACTATTGTT | |||
AK18 | ERR7598207.14365408 | (GTC)7 | F: CGATGTGGCCTTTTGTCG R: CTTCCAAAATTTCATAAAACACTGC | |||
AK19 | ERR7598207.14367708 | (CA)7 | F: CAACTGAAGAGTGAATTCCAAAA R: AGGGCGATACGGTCAAAAT | |||
AK20 | ERR7598207.8923957 | (CG)10 | F: GGTTTTCCCCGAGTTCGT R: GGGTGGGTGGGTTAATTTTC | |||
AK21 | ERR7598207.13745169 | (AATT)5 | F: GCTTTTTATCTGGTGAAATGCT R: AGCAGCAACACCATCATGC | |||
AK22 | ERR7598207.14102450 | (GGAA)5 | F: CGACTCGGTACGAGTTCACA R: CAACCGAACCAACCAATAGT | |||
AK24 | ERR7598207.14368656 | (CT)17 | F: GATTTCGAAACATGGTGAAAG R: GATGTAGCCATGATTGCAAGTAG | |||
AK25 | ERR7598207.14357143 | (GA)12 | F: AACTGTTCGCAATTGGCTTT R: ATTCATAGCACTCGGCGAAA | |||
AK26 | ERR7598207.14364132 | (TG)8 | F: CGCTCCGATTTTCGTATTCA R: CGTCGGGCTCAGACTATTTG | |||
AK27 | ERR7598207.14359268 | (TA)7 | F: GTCGGTGATTGTCACCATGT R: CATCCAGAGTGCATCAATCG | |||
AK29 | ERR7598206.10239627 | (GGCC)4 | F: CTACCCTTGCTTGGAGGTTG R: GTCGAGACGTGTGAGAGTGC | |||
AK30 | ERR7598207.11336441 | (GC)5 | F: TGAGTAACTGCGAGCTTGTCTC R: GAGATTGATTGTAAATACACACACACA | |||
AK31 | ERR7598207.14342765 | (TGA)6 | F: TCGCTGGAATGGTATAAGGAA R: TTGCCTTGCTACATTAGATGGT |