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Fig. 3 | Parasites & Vectors

Fig. 3

From: Identifying the function of genes involved in excreted vesicle formation in Acanthamoeba castellanii containing Legionella pneumophila

Fig. 3

Legionella in siRNA-transfected Acanthamoeba. siRNA-transfected A. castellanii which ingested L. pneumophila for 12 h (siRNA-A+L). Cells were stained with Giemsa staining solution. a ACA1_114460 siRNA-transfected A. castellanii Castellani, b ACA1_091500 siRNA-transfected A. castellanii Castellani, and c ACA1_362260 siRNA-transfected A. castellanii Neff. d Quantification of excreted vesicles following siRNA treatment. Arrows: excreted vesicles containing Legionella, black arrowheads: free legionellae, and white arrowheads: excreted vesicles containing Legionella. A Acanthamoeba only, A+L Acanthamoeba with L. pneumophila, siRNA-A siRNA-transfected Acanthamoeba, and siRNA-A+L siRNA-transfected Acanthamoeba co-cultured with L. pneumophila. Images were acquired at ×1000 magnification. Asterisks denote statistical significance compared to A+L control (*** P < 0.001, **** P < 0.0001)

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Parasites & Vectors

ISSN: 1756-3305

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