Fig. 6

Passive immunization protects against P. berghei infection in BALB/c mice induced by Bm8 antisera. a Detection of the copy number of P. berghei gene in BALB/c mice on days 3, 6, 9 and 12 post infection by qRT-PCR. Asterisk indicates significant difference at *P < 0.05 vs adjuvant group (t-test). b Parasitemia comparison in BALB/c mice infected with P. berghei on days 3, 6 and 9 post infection versus control group detected by microscopy. Asterisk indicates significant difference at *P < 0.05 vs adjuvant group (t-test). c Severity of mice babesiosis in mice passive immunization with Bm8 polypeptides antisera group verus those receiving control sera: i, ii, iii changes in Hb level (i), body weight (ii) and body temperature (iii) in BALB/c mice challenge infection with P. berghei days 3, 6 and 9 post infection. a, b, c (i). Asterisks indicate a significant difference at ***P < 0.001 (t-test). Bm8, B. microti protein 8; Hb, hemoglobin; qRT-PCR, real-time quantitative PCR; mRNA, messenger RNA; real-time quantitative PCR