Fig. 2

MiR-3976 regulates the expression of BCL2A1. a Binding sites of miR-3976 to BCL2A1 predicted by TargetScan. b Relative BCL2A1 expression levels in HCT-8 cells at different time points after C. parvum infection by RT-qPCR. c Binding of miR-3976 to BCL2A1 detected by dual-luciferase activity in HCT-8 cells co-transfected with miR-3976 mimics or miR-3976 NC and pmirGLO-BCL2A1-WT or pmirGLO-BCL2A1-MUT for 48 h. The left represents the luciferase reporter assay in HCT-8 cells co-transfected with miR-3976 and BCL2A1, and the right represents sequences of BCL2A1-WT and BCL2A1-MUT. d and e Cells were transfected with miR-3976 mimics and miR-3976 inhibitor for 24 h and then exposed to equal numbers of C. parvum sporozoites for 24 h. BCL2A1 mRNA and protein expression levels were detected by RT-qPCR and western blotting. The densitometric levels of BCL2A1 protein signals and mRNA levels were quantified and expressed relative to GAPDH. All data presented as combined mean ± SD of three independent experiments. Different groups were compared by t-tests. *P < 0.05, **P < 0.01, ***P < 0.001