Fig. 3

MiR-3976 directly targets BCL2A1 to regulate cell apoptosis and parasite burden in HCT-8 cells after C. parvum infection. a Expression efficiency of BCL2A1 in HCT-8 cells transfected with si-BCL2A1 and pcDNA3.1-BCL2A1 for 24 h by RT-qPCR. b Effect of BCL2A1 on cell apoptosis infected with C. parvum. Cells were transfected with si-BCL2A1 and pcDNA3.1-BCL2A1 for 24 h and then exposed to equal numbers of C. parvum sporozoites for 24 h. Cell apoptosis was then detected by flow cytometry. c Regulation of parasite burden in HCT-8 cells by BCL2A1. Cells were transfected with si-BCL2A1 and pcDNA3.1-BCL2A1 for 24 h and then exposed to equal numbers of C. parvum sporozoites for 2 h to determine the initial cellular invasion of C. parvum by RT-qPCR. Simultaneously, infected cells were cultured for 48 h to evaluate the parasite burden in cells after initial cell invasion. d Expression efficiency of miR-3976 in HCT-8 cells co-transfected with miR-3976 mimics and pcDNA3.1-BCL2A1 for 24 h by RT-qPCR. e Effect of miR-3976 targeting BCL2A1 on apoptosis of cells infected with C. parvum. Cells were co-transfected with miR-3976 mimics and pcDNA3.1-BCL2A1 for 24 h and then exposed to equal numbers of C. parvum sporozoites for 24 h. Cell apoptosis was detected by flow cytometry. f Regulation of miR-3976 targeting BCL2A1 on parasite burden in HCT-8 cells. Cells were co-transfected with miR-3976 mimics and pcDNA3.1-BCL2A1 for 24 h and then exposed to equal numbers of C. parvum sporozoites for 2 h to determine the initial cellular invasion of C. parvum by RT-qPCR. Simultaneously, infected cells were cultured for 48 h to evaluate the parasite burden in cells after initial cell invasion. All data presented as combined mean ± SD of three independent experiments. Different groups were compared by t-tests. *P < 0.05, **P < 0.01, ***P < 0.001