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Table 2 Molecular detection of Cryptosporidium hominis in invertebrate larvae samples seeded with approximately 200 oocysts

From: The occurrence and zoonotic potential of Cryptosporidium species in freshwater biota

Sample

Number of replicates

Number of oocysts seeded

Mean Cta (± SD)

Number of Cryptosporidium-positive samples

Baetis sp.

3

200

36.37 (± 1.20)

2

Diplectrona sp.

3

200

34.94 (± 1.38)

3

Simulium sp.

3

200

37.88 (± 0.52)

3

Amphinemura sp.

3

200

38.45 (± 0.48)

2

Leuctra sp.

3

200

37.05 (± 0.06)

2

Rhithrogena sp.

3

200

36.68 (± 1.77)

3

Baetis sp. Control

1

200

35.12

1

Negative control

1

0

Negative

0

C. hominis Control

1

200

33.36

1

  1. Ct Threshold cycle (number of cycles required to detect fluorescence of a sample in real-time quantitative PCR, SD standard deviation