Table 3 Molecular detection of Cryptosporidium hominis in invertebrate larvae samples seeded with approximately 5, 25, 50 or 100 oocysts
From: The occurrence and zoonotic potential of Cryptosporidium species in freshwater biota
Sample | Number of replicates | Number of oocysts seeded | Mean Ct (± SD) | Number of Cryptosporidium-positive samples |
|---|---|---|---|---|
Baetis sp. | 1 | 100 | 36.60 (± 1.73) | 3 |
Baetis sp. | 1 | 50 | 36.24 (± 1.86) | 3 |
Baetis sp. | 1 | 25 | Negative | 0 |
Baetis sp. | 1 | 5 | 38.44 (± 0.18) | 1 |
Amphinemura sp. | 1 | 100 | 35.28 (± 0.18) | 3 |
Amphinemura sp. | 1 | 50 | 37.98 (± 1.85) | 1 |
Amphinemura sp. | 1 | 25 | 37.56 | 1 |
Amphinemura sp. | 1 | 5 | 37.4 | 1 |
Negative control | 1 | 0 | Negative | 0 |
C. hominis Control | 1 | 200 | 32.92 | 1 |