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Fig. 6 | Parasites & Vectors

Fig. 6

From: Comparative transcriptome profiling of Eimeria tenella in various developmental stages and functional analysis of an ApiAP2 transcription factor exclusively expressed during sporogony

Fig. 6

Construction, and phenotyping of ETH2_0411800 overexpression parasites. A Schematic representation of ETH2_0411800 gene-overexpressing vector. Two Flag peptides (in red) were fused at the N and C terminal. B The expression of ETH2_0411800 (fused upstream of EYFP) in oocysts was determined by fluorescent microscopy. C Western blotting showing ETH2_0411800 expression. D IFA analysis of the ETH2_0411800 protein in overexpression parasites by using an anti-Flag antibody. The nuclei were visualized by Hoechst staining. E Oocyst output curves and F total oocyst output of overexpression strain. Chicken (n = 3) were infected with 1000 oocysts of the overexpression strain. ETH was used as a control. Error bars represent SEM. P-values were obtained using unpaired two-tailed Student’s t-tests. *P < 0.05; **P < 0.01; ***P < 0.001; ns not significant

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Parasites & Vectors

ISSN: 1756-3305

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