Table 1 Detailed information on primers used for amplifying different target regions by polymerase chain reaction (PCR) in the present study
Gene | Primer name | Primer sequence (5′ → 3′) | References |
|---|---|---|---|
18S | 18S-F | CGCGAATRGCTCATTACAACAGC | [46] |
18S-R | GGGCGGTATCTGATCGCC | ||
28S | 28S-F | AGCGGAGGAAAAGAAACTAA | [47] |
28S-R | ATCCGTGTTTCAAGACGGG | ||
ITS1-5.8S | SS1 | GTTTCCGTAGGTGAACCTGCG | [48] |
SS2R | AGTGCTCAATGTGTCTGCAA | ||
ITS2 | NC13 | ATCGATGAAGAACGCAGC | [48] |
NC2 | TTAGTTTCTTTTCCTCCGCT | ||
cox1 | NTF | TGATTGGTGGTTTTGGTAA | [49] |
NTR | ATAAGTACGAGTATCAATATC | ||
cox2 | COII-F | AATTTTAATTGTAGTCTTTTGTTTGG | [50] |
COII-R | CTATGATTAGCACCACAAATC | ||
12S | 12S-F | GTTCCAGAATAATCGGCTA | [51] |
12S-R | ATTGACGGATGGTTTGTACC |