Fig. 4

Differential diagnosis of B. crassa-like and B. motasi hebeiensis. 1C PCR product of plasmid of B. crassa-like, 2C PCR product of plasmid of B. motasi Hebei, 1 mixed samples of PCR products of plasmid and DNA of B. crassa-like, 2 mixed sample of PCR product of B. motasi hebeiensis DNA and PCR product of B. crassa-like plasmid. The qPCR product of the plasmid of B. crassa-like was mixed with the qPCR products of two unknown Babesia samples at a volume of 1:1, and then HRM analysis was performed