Table 4 Comparison of the performance of microscopy, nested real-time PCR and the newly developed real-time PCR–high-resolution melting method for the detection of B. duncani
Results | Detection method | ||
|---|---|---|---|
Microscopy | qPCR-HRM | Nested-qPCR | |
True positive | 35/40 | 39/40 | 40/40 |
False positive | 2 | 0 | 0 |
True negative | 38/40 | 160/160 | 160/160 |
False negative | 5 | 1 | 0 |
Specificity (%) | 95 | 100.0 | 100.0 |
Sensitivity (%) | 87.5 | 97.5 | 100.0 |