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Fig. 2 | Parasites & Vectors

Fig. 2

From: First report on knockdown resistance mutations in wild populations of Aedes aegypti from Argentina determined by a novel multiplex high-resolution melting polymerase chain reaction method

Fig. 2

a–e Results of genotyping positions 1016 and 1534 in the voltage-gated sodium channel (Nav) gene of Aedes aegypti (aedaenav) with high-resolution melting (HRM). Detection of kdr single nucleotide polymorphism by melt curve analysis. Alleles are distinguished by changes in the melting temperature (MT). a Raw results of multiplex HRM (mHRM). Peaks on the left and on the right indicate, respectively, IIS6 and IIIS6 Nav segment amplicons. b, c Derivative and difference melting plots for IIS6 with variation in the 1016 position. d, e Derivative and difference melting plots for IIIS6 with variation in the 1534 position. Red kdr homozygous standard (R2R2), yellow heterozygous standard (SR2), green wild type homozygous standard (SS)

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Parasites & Vectors

ISSN: 1756-3305

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