Table 2 Comparison of methods for the genotyping of kdr mutations in positions 1016 and 1534 (genotyping of 85 individuals for both positions)
Multiplex allele-specific PCRa | TaqManb | Singleplex HRM | Multiplex HRM | |
|---|---|---|---|---|
Equipment required | Thermocycler; electrophoresis system; transilluminator with camera; computer | Real-time thermocycler; computer | Real-time thermocycler; computer | Real-time thermocycler; computer; software |
Equipment costsc (USD) | 9500 | 20,000 | 20,000 | 21,000 |
Time for completion | 4.5 h | 2.5 h | 2.5 h | 1.2 h |
Characteristics | PCR followed by gel electrophoresis; multiplex | Closed tube; one independent reaction for each mutation | Closed tube; one independent reaction for each mutation | Closed tube; multiplex |
Number of steps | 2 | 2 | 2 | 1 |
Number of primers | 7 | 4 | 4 | 4 |
Number of TaqMan probes | 0 | 4 | 0 | 0 |
Number of tubes/wells required | 96 | 192 | 192 | 96 |
Running costs (USD) | 40 | 212 | 129 | 64 |