Fig. 2
Metabolomic profiling of miR-1174-depleted mosquitoes. a Score scatter plot of PCA model for groups with quality control. All samples were within the 95% confidence interval (Hotelling's T-squared ellipse). b Score scatter plot of OPLS-DA model for Ant-1174 vs Control. c Permutation test of OPLS-DA model between Ant-1174 and control groups. Intercepts: R2Y(cum) = (0, 0.79), Q2(cum) = (0, − 0.97). d Volcano plot of differential metabolites. Red dots represent upregulated metabolites, blue dots are downregulated ones. The X-axis indicates log2 fold change, and the Y-axis measures the − log10 P-value. e Heatmap of differential metabolites. Downregulated metabolites are highlighted in blue and clustered in the upper left, upregulated metabolites are represented in red and clustered in the lower left. f Classification of upregulated metabolites and downregulated metabolites. g KEGG pathways enriched by the differential metabolites. The circle size is directly proportional to the number of differential metabolites; the depth of red is inversely proportional to the P-value. h Metabolic pathway analysis of differential metabolites. In the bubble diagram, each bubble represents a metabolic pathway, and the size of the bubble represents the influence factor size of the pathway in topological analysis. Ant-1174, Antisense oligonucleotide (antagomirs) Ant-1174; KEGG, Kyoto Encyclopedia of Genes and Genomes; miR-1174, microRNA-1174; OPLS-DA, orthogonal projections to latent structures- discriminant analysis; PCA, principal component analysis