Fig. 3

Target genes of miR-1174. a Predicted binding site within the 3’-UTR of serine protease inhibitor 27A (SPI27A, AAEL014079). b Dual-luciferase reporter assay of the binding site within the 3’-UTR of SPI27A mRNA. c Predicted binding site within the 3′-UTR of MCT12 mRNA. d Dual-luciferase reporter assay of the binding site within the 3′UTR of MCT12 mRNA. e Predicted binding site within the 3’-UTR of uncharacteristic (UC, AAEL015019). f Dual-luciferase reporter assay of the binding site within the 3′UTR of UC mRNA. g Dual-luciferase reporter assay to check whether miR-1174 targets the 5’-UTR of PNP mRNA. h Dual-luciferase reporter assay to check whether miR-1174 targets the CDS of PNP mRNA. i Dual-luciferase reporter assay to check whether miR-1174 targets the 3’UTR of PNP mRNA. Error bars depict ± SEM of three independent experiments. Asterisks indicate statistical significance at *P < 0.05 and **P < 0.01; ns, not significant. CDS, Coding sequence; miR-1174, microRNA-1174; UTR, untranslated region; mRNA, messenger RNA; PNP, purine nucleoside phosphorylase; SEM, standard error of the mean; 3′-UTR, 3′-untranslated region