Fig. 5

Expression of PNP. a RT-qPCR-based temporal expression of PNP in adult female mosquitoes. b Western blotting analysis of PNP in adult mosquitoes. In order to compare with the mRNA levels in a, the signals of the western blotting results (below) were converted into numbers using Image J software to quantify protein expression (above). c RT-qPCR-based spatial expression at 72 h PE. d RT-qPCR-based spatial expression at 6 h PBM. e Expression of PNP in response to miR-1174 depletion. Females were dissected in phosphate-buffered saline for tissue collection, and the blood boluseswere removed from the midgut. RPS7 was used as the control for RT-qPCR, and tubulin was used as the control for western blotting. All graphs are presented as mean ± SEM of 3 independent experiments. Differences at P < 0.05 were statistically significant, and differences at P < 0.01 were considered to be highly statistically significant. FB, Fat body; HD, head; LO, leftover; Mg: midgut; miR-1174,, microRNA-1174; PBM, post blood meal; PE, posteclosion; PNP, purine nucleoside phosphorylase; RT-qPCR, real-time quantitative PCR; SEM, standard error of the mean