Fig. 6

Abnormal phenotypes caused by PNP knockdown. a RT-qPCR results showing the knockdown of PNP at 72 h PE. b RT-qPCR results showing the knockdown of PNP at 6 h PMB. c Western blotting results showing the knockdown of PNP at 72 h PE. d Western blotting results showing the knockdown of PNP at 6 h PBM. e Phenotypes caused by PNP RNA interference. f Statistics of egg laying (N = 10 females × 6 biological replicates). g Statistics of blood sucking rate (N = 40 females × 4 biological replicates). h. Statistics of survival rate at 72 h PBM (N = 60 females per group × 3 replicates). RPS7 was used as the control for RT-qPCR, and dsEGFP was used as the control for RNA interference. Error bars depict ± SEM of three independent experiments. Differences at P < 0.05 were considered to be statistically significant, and differences at P < 0.01 were considered to be highly statistically significant. dsEGFP, Double-stranded RNA of enhanced green fluorescent protein; dsPNP, double-stranded RNA of purine nucleoside phosphorylase; PBM, post blood meal; PE, posteclosion; RT-qPCR, real-time quantitative PCR; RPS7, ribosomal protein S7; SEM, standard error of the mean; WT, wild type