Fig. 7

Knockdown of PNP in mosquitoes changes the expression of some signaling pathway genes and affects energy metabolism. Depicted are expression of the following. a Vitellogenin gene (Vg). b Ecdysteroid receptor gene (EcR). c Ecdysone-induced protein 75A gene (E75A). d Ecdysone-induced protein 74B gene (E74B). e Mosquito insulin receptor gene (MIR). f Phosphoinositide 3-kinase gene (PI3K). g Target of rapamycin gene (TOR). h Death regulator Nedd2-like caspase gene (Dronc). i Autophagy related 8 gene (ATG8). j ATP level at 36 h PBM. k TG level at 72 h PE without a blood meal. l TG level at 36 h PBM. Data are presented as the mean ± SEM of 3 independent experiments. Statistical comparisons between groups were performed using a one-way ANOVA followed by an unpaired Student’s t-test. Differences at P < 0.05 were considered to be statistically significant, and differences at P < 0.01 were considered to be highly statistically significant. ANOVA, Analysis of variance; dsEGFP, Double-stranded RNA of enhanced green fluorescent protein; dsPNP, double-stranded RNA of purine nucleoside phosphorylase; RPS7, ribosomal protein S7; TG, triglyceride; WT wild type