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Fig. 2 | Parasites & Vectors

Fig. 2

From: RNAi-mediated CHS-2 silencing affects the synthesis of chitin and the formation of the peritrophic membrane in the midgut of Aedes albopictus larvae

Fig. 2

Expression patterns of CHS-2 gene in Ae. albopictus. Real-time quantitative fluorescence PCR (qPCR) was used to detect the relative expression level of CHS-2 in different periods of Ae. albopictus and in different tissues of the fourth-instar larvae of Ae. albopictus. A Relative expression of CHS-2 in Ae. albopictus at different stages: egg, third-instar larva (3rd); fourth-instar larva (4th); adult Ae. albopictus (female) at 24 h after eclosion (AD1); adult Ae. albopictus (female) at 48 h after eclosion (AD2); adult Ae. albopictus (female) at 24 h after blood-sucking (AD3); adult Ae. albopictus (female) at 48 h after blood-sucking (AD4). Different lowercase letters above the bar indicate that the difference is statistically significant (one-way ANOVA, Tukey test, P < 0.05). B Relative expression of CHS-2 in the midgut and cuticle of fourth-instar larvae. Relative expression levels were calculated in comparison with that at 4th (A) and cuticle (B), which were ascribed an arbitrary value of 1. Three biological replicates and three technical replicates were set. (One-way ANOVA, Duncan test, *P < 0.05, **P < 0.01)

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Parasites & Vectors

ISSN: 1756-3305

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