Fig. 4From: RNAi-mediated CHS-2 silencing affects the synthesis of chitin and the formation of the peritrophic membrane in the midgut of Aedes albopictus larvaeSurvival and development of fourth-instar larvae of Ae. albopictus after CHS-2 gene silencing. Fifty larvae of the fourth-instar Ae. albopictus were randomly selected and fed normally after injection of dsRNA. The mortality rate of larvae after 24Â h and 48Â h (A) and the pupation rate and emergence rate of larvae within 72Â h (B) were recorded. Three biological replicates and three technical replicates were set. (One-way ANOVA, Duncan test)Back to article page