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Table 2 Primers for synthesis of dsRNA

From: RNAi-mediated CHS-2 silencing affects the synthesis of chitin and the formation of the peritrophic membrane in the midgut of Aedes albopictus larvae

Primer name

Forward primer (5′-3′)

Reverse primer (5′-3′)

dsCHS-2

GGATCCTAATACGACTCACTATAGGAGTCAACGCACCGTACAGGA

GGATCCTAATACGACTCACTATAGGGAAGAGCAACACCAAACCGA

dsGFP

GGATCCTAATACGACTCACTATAGGAAGGGCGAGGAGCTGTTCACCG

GGATCCTAATACGACTCACTATAGGCAGCAGGACCATGTGATCGCGC

  1. The underlined is the T7 promoter