Fig. 3

Synthetic odour blends of aquatic stage-conditioned water regulate oviposition choice and egg-laying in Aedes aegypti. The dose-dependent oviposition choice by gravid mosquitoes to individual synthetic odour blends identified as associated with water conditioned with a eggs, b second-instar larvae, c fourth-instar larvae and d pupal exuviae, in comparison with a solvent control (hexane). Asterisks indicate significant differences (P < 0.05), as determined by a nominal logistic general regression analysis followed by an odds ratio comparison. Gravid Ae. aegypti were differentially stimulated to lay eggs in response to these treatments (e–h). The different lowercase letters represent significant differences (P < 0.05), as determined by an ANOVA followed by a Tukey post-hoc test. Error bars represent the standard error of the mean. The sample size is greater than 30 for all comparisons