Fig. 2
From: CRISPR/Cas12a combined with RPA for detection of T. gondii in mouse whole blood
Sensitivity of the DETECTR system: (a) B1-DETECTR and (b) 529 RE-DETECTR reaction with plasmid standards. The tube shows the fluorescence signal of the sample under UV light. 1–8 represent 3000 cp/μl, 300 cp/μl, 30 cp/μl, 3 cp/μl, 1.5 cp/μl, 0.3 cp/μl, 0.03 cp/μl, negative control. c B1-DETECTR and d529 RE-DETECTR reaction with T. gondii tachyzoite DNA. e B1-nested PCR and f 529 RE-nested PCR reaction with T. gondii tachyzoite DNA. 1–7 represent 10,000, 1000, 100, 10, 1, 0.1, 0.01 tachyzoite DNA per reaction. ****P < 0.0001; ns, not significant