Fig. 3

GO term annotation and KEGG pathway enrichment of significant DEGs between in vitro and in vivo groups. A Volcano plot of relative mRNA expression from B. gibsoni (Wuhan isolate) continuous in vitro cultured parasites compared to in vivo original parasites. Genes at left (blue) and right (yellow) are downregulated and upregulated DEGs, respectively. B GO term annotation of significant DEGs. C KEGG pathway enrichment of screened significant DEGs