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Fig. 9 | Parasites & Vectors

Fig. 9

From: Transcriptional variation in Babesia gibsoni (Wuhan isolate) between in vivo and in vitro cultures in blood stage

Fig. 9

Immunoblotting of native BgAP2-M protein from in vitro cultured or in vivo B. gibsoni (Wuhan isolate). Lane M: molecular weight marker; Lane 1: lysate of in vitro cultured B. gibsoni probed with rabbit polyclonal antibodies against BgAP2-M peptides; Lane 2: lysate of in vivo B. gibsoni probed with rabbit polyclonal antibodies against BgAP2-M peptides; Lane 3: lysate of noninfected Canis erythrocytes probed with rabbit polyclonal antibodies against BgAP2-M peptides; Lane 4: lysate of in vitro cultured B. gibsoni probed with pre-immune rabbit serum; Lane 5: lysate of in vivo B. gibsoni probed with pre-immune rabbit serum; Lane 6: lysate of noninfected Canis erythrocytes probed with pre-immune rabbit serum. Arrows indicate the corresponding bands. Anti-histone H3 antibody was used as a loading control to detect the expression of BgAP2-M

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Parasites & Vectors

ISSN: 1756-3305

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