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Fig. 2 | Parasites & Vectors

Fig. 2

From: High-resolution melting (HRM)-based detection of polymorphisms in the malic enzyme and glucose-6-phosphate isomerase genes for Leishmania infantum genotyping

Fig. 2

Sensitivity curves of the qPCR-ME65 assay (a) and qPCR-GPI88 assay (b). The standard curves were spiked with 30 ng of human or canine DNA (upper curves partially overlapping, circles and triangles points, respectively) or non-spiked (lower curve, square points). Each point represents duplicate result of two independent experiments. Cq, Quantification cycle; GPI, glucose-6-phosphate isomerase; ME, malic enzyme; qPCR, quantitative real-time PCR

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Parasites & Vectors

ISSN: 1756-3305

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