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Fig. 3 | Parasites & Vectors

Fig. 3

From: High-resolution melting (HRM)-based detection of polymorphisms in the malic enzyme and glucose-6-phosphate isomerase genes for Leishmania infantum genotyping

Fig. 3

HRM analysis of Leishmania infantum reference strains. a, b qPCR-ME65 HRM negative derivative plots (a) and normalized profiles (b) were obtained by using DNA from MHOM/TN/80/IPT1 (genotype 390G) and MHOM/FR/78/LEM75 (genotype 390T). c, d qPCR-GPI88 HRM negative derivative plots (c) and normalized profiles (d) were obtained by using DNA from MHOM/TN/80/IPT1 (genotype 1831A), MHOM/FR/78/LEM75 (genotype 1831G) and MHOM/IT/93/ISS822 (genotype 1831R). dF/dT, derivative of the intensity of fluorescence at different temperatures; GPI, Glucose-6-phosphate isomerase; HRM, high-resolution melting; ME, malic enzyme; qPCR, quantitative real-time PCR; T, temperature

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Parasites & Vectors

ISSN: 1756-3305

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