Skip to main content
Fig. 4 | Parasites & Vectors

Fig. 4

From: High-resolution melting (HRM)-based detection of polymorphisms in the malic enzyme and glucose-6-phosphate isomerase genes for Leishmania infantum genotyping

Fig. 4

HRM analysis of Leishmania infantum clinical samples. a, b qPCR-ME65 HRM negative derivative plots (a) and normalized profiles (b) of selected clinical samples. As references, MHOM/TN/ 80/IPT1 (genotype 390G) and MHOM/FR/78/LEM75 (genotype 390 T) strains were included in the assay. c, d qPCR-GPI88 HRM negative derivative plots (c) and normalized profiles (d) of selected clinical samples. As references, MHOM/TN/80/IPT1 (genotype 1831A), MHOM/FR/78/LEM75 (genotype 1831G) and MHOM/IT/93/ISS822 (genotype 1831R) strains were included in the assay. dF/dT, derivative of the intensity of fluorescence at different temperatures; HRM, High-resolution melting; GPI, glucose-6-phosphate isomerase; ME, malic enzyme; qPCR, quantitative real-time PCR

Back to article page

Parasites & Vectors

ISSN: 1756-3305

Contact us