High-resolution melting (HRM)-based detection of polymorphisms in the malic enzyme and glucose-6-phosphate isomerase genes for Leishmania infantum genotyping

Table 3 Polymorphisms identified in samples sequenced by multilocus sequence typing, compared to Leishmania infantum JPCM5 genome GCA_900500625.2

Protein-coding genes^a	Sample ID^b
Protein-coding genes^a	Pan-30	Pan-42	MHOM/TN/80/IPT1	MHOM/FR/78/LEM75	Pan-64	MHOM/IT/2019/Cur-1	MHOM/IT/08/31U	Bra-aii	Elr-sci
Elongation initiation factor 2alpha LINF_030014900
Spermidine synthase LINF_040010800
Isocitrate dehydrogenase LINF_100008300						130806C 131265Y 131511T
Glucose-6-phosphate isomerase LINF_120010600	92519A	292519A	292519A 2292809A	292519A	292519A 2292809A	292519A 2292809R	292519A	292519A 2292809A	292519A
Inosine-guanine nucleoside hydrolase LINF_140006200						32,800 T
Nonspecific nucleoside hydrolase LINF_180021400						686,833 G
Malic enzyme LINF_240012800			281164G			280978Y 281103G 281164G 281281G 281617Y 281693R		281164G
Phosphomannose isomerase LINF_320021600						621512
Glucose-6-phosphate 1-dehydrogenase LINF_340005700						26839S 26878C 27181C 27947R
Malate dehydrogenase LINF_340006400						47256R 47877C 48169M 48225G
Arginase LINF_350019900						571026M
6-Phosphogluconate dehydrogenase LINF_350038800						1288246C
Phosphomannomutase LINF_360026300						783228S
UDP-N-Acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase LINF_360051000

Italics indicate the position of polymorphisms 1831A/G on the glucose-6-phosphate isomerase gene (nucleotide 292809 on chromosome 12) and polymorphisms 390T/G on the malic enzyme gene (nucleotide 281164 on chromosome 24) on which the high-resolution melting assays have been designed
^aFourteen protein-coding genes were selected for the multilocus sequence typing (MLST) assay
^bSee Table 1 for a full description

ISSN: 1756-3305