Table 1 List of human-specific primers and thermal cycles used in the study

Alu-repeats

F: CGAGGCGGGTGGATCATGAGGT

R: TCTGTCGCCCAGGCCGGACT

226

94 °C for 5 min; followed by 35 cycles of 94 °C for 25 s, 64.4 °C for 25 s and 72 °C for 20 s; with a final extension at 72 °C for 5 min

[39]

Cytb

F: TTCGGCGCATGAGCTGGAGTCC

R: TATGCGGGGAAACGCCATATCG

228

94 °C for 2 min; followed by 35 cycles of 94 °C for 30 s, 64 °C for 40 s and 72 °C for:30 s; with a final extension at 72 °C for 8 min

[45]

16S rRNA

F: CAATTGGACCAATCTATCACC

R: GTGAGGGTAATAATGACTTGT

157

94 °C for 5 min; followed by 35 cycles at 95 °C for 25 s, 65.8 °C for 25 s and 72 °C for 20 s; with a final extension at 72 °C for 5 min

[46]